Cognitive Framing Theory and Spiral or Silence Research Paper - 1

Cognitive Framing Theory and Spiral or Silence - Research Paper Example The thinking and all that matters in talking does involve framing. This is the basis of cognitive framing theory. According to Lakoff (2010), a single word defines not only the word, but it activates the frames by defining much about the system that is in it. However, frames do not work in solitude but they have a direct connection to all part of the brain including the emotional regions (Lakoff, 2010). This explains why people have different taste to different circumstances. The workings of the frame make it possible for some people to like football while other dislikes the same. Thus, it is how best a frame is internalized that explains the behaviors of individuals and emotional reactions to their surroundings. The political scene is one good example where systems of frames are vehemently used. Members of political parties share the political ideologies, and they all adhere to this course. Therefore, politician uses a language that point to specific ideological systems. The process they go through whether in party fundraising or conventional nomination informs their subconscious of the principle that they defend. The development of this political ideology frames explains the subsequent behaviors. Cognitive framing theory also argues that ideological language becomes a normal language as it unconsciously activates the brain of the ideas. Therefore, people cannot avoid framing, and they can only be strengthened or weakened. There are different frames and individual choose among the many in any given situation. Moreover, there are limited ways of changing frames. The thing that a person has made cognates in the brain can only relate to news set of frames that relate to the existing systems of frames (Lakoff, 2010). The changes need to be emotional and introduced through a communication system that must be sufficient, repetitive, and full of trust for the messengers. Negating frames tend to activate

Hispanic American Diversity Essay Example | Topics and Well Written Essays - 1250 words - 1

Hispanic American Diversity - Essay Example People of Mexican origin are Mexicans either for the reason they are born in Mexico or of Mexican heritage. "The majority of Mexican Americans live in urban areas which comprise the three agricultural migrant streams that flow from the south to the north across the country, often twice annually. Historically, Mexican Americans have been both an urban and rural population. Since the 1600s, Mexicans were the first Americans to establish homesteads in the territories that became Arizona, California, Colorado, New Mexico, and Texas. Since before the turn of the century, Mexican Americans literally built the great southwestern cities of Los Angeles, San Diego, Tucson, Albuquerque, Dallas, and San Antonio" (2006b) Mexican culture is influenced by folk art, which exhibits in major American museums, and "the great Mexican muralists like Rivera, Siquieros, and Orozco were invited to paint frescoes on the walls of American institutional sites such as universities and even within such edifices of capitalism as Rockefeller Center in New York" (Limon, 1998, p. 26) Local Mexican cultural life involves the participation of ranches, they used to gather for parties and seasonal balls and dutifully attend Sunday gatherings. With respect to linguistics, "Mexican America... Others, speaking perfect English, have completely forsaken the tongue of their ancestors". (Gonzalez, 1969, p. 16) A few, usually among the more educated intellectual class, have made a point of learning to speak both English and Spanish well, but they are, very rare. It has been found that the majority of the members of this population still learn English as a second language, however Spanish-American leaders recognize that in order for individuals to raise their socioeconomic level they must speak English well. Puerto Ricans The cultural heritage of Puerto Rico is among the richest in the world. Traditionally, the Puerto Rican people speak Spanish; it is their language of loyalty, romance, passion, patriotism, business, negotiation, and defiance-it is their cultural language. Their ancestors inherited this language from the Spaniards through conquest, and yet it is not the language that the Spaniards spoke when Puerto Rico was under Spanish colonial rule, and it is not the Spanish that Spaniards speak today. In addition, for those who are fully bilingual in Spanish and English, a linguistic phenomenon called "Spanglish" has arisen. The Spanish conquest of Puerto Rico, and the rest of Latin America, for the most part guaranteed that the people would become Roman Catholic. Under colonial rule, it was forbidden to practice any religion but Catholicism. "In Puerto Rico there seems to be a combination of the practices of Santeria and Spiritism, and there are times when the people themselves are not certain o f the origin of a particular custom; they practice it because their ancestors did, not because they are active followers of Santeria or Spiritism". (Perez, 2000, p. 17) Politically, "Puerto Ricans have always received lower levels of Federal

Culture’s will to copy Essay Example for Free

Culture’s will to copy Essay Globalization process is viewed as a means through which one can ratify often in extremely idealized form a account of oneself or culture that is observed as old or even origin but can lastly be realized: through these new means, one can become what one thinks one actually is (even if one never was). What might be trait of the Internet is that this ‘realization’ is certainly ‘expansive’. Globalization process has an emancipator technology ‘Internet’ that is indefensible as the structural design of the technology harbors an instinctive class prejudice and other shades of power entitlements. Computers are intended and programmed by members of the elite culture and might imitate their cultural orientations and biases. For example, the wordsmith and semantic skills requisite to functions computers do not put up the cultural orientations of several marginal electorates. As Laikwan Pang, Cultural Control in journal said, â€Å"Culture’s will to copy [is] fuelled by the globalization process, which drives’ the world to desire similar but different products, to acquire similar but different tastes†. (Laikwan Pang, Cultural Control, p8). Globalization is as well redefining societies and restructuring society into new forms of social networks. New standards and terms for private and proficient relationships are promising (Buck 1996; Gates 1995; Baym 1995). The London Times (June 17, 1996) stated: People in every kinds of career categories need to recognize how to use this tool so as to get ahead starting now. Admittance to the information freeway might establish to be less a question of dispensation or position than one of the fundamental capability to function in a democratic society. Admittance to the cyberspace might very well establish how well people are knowledgeable, the type of job they ultimately get, and how they are retrained if they mislay their job, how much access they have to their government and how they will be taught about important issues concerning them and the country. (Ratan 1995: 25) Moreover, global media is not repressed by the intrinsic biases apparent in sexism, racism, and classism establish in face-to-face encounters. As a substitute, the global media presents a discussion that supports broad partaking and underlines merit over class. Practical communities permit secluded individuals to converse in a manner that protects them from the social prospect and sanctions linked with physically distinct communities (Turtle 1995). Virtual societies are unified and significant social aggregations that permit people to take on in adequate relations to form personal and group relations (Rheingold 1993). Global media represents Hollywood that spins around the analysis of Hollywoods division of labor, what the authors call the New International Division of Cultural Labor (NICL). This division of labor is certainly international because U. S. film exports have reached $11 billion, and Hollywoods proportion of the world market is double what it was in 1990 (Miller et al. , 2001, pp. 4-5). Global sales have become so significant that in 2001 the studios take apart their international offices to run all global distribution from their headquarters. The authors argue that Hollywoods command of the NICL distinguishes Hollywood from other industries that are increasingly globalizing. The entire book focuses on answering this question: Is Hollywood really giving the people of the world what they want, or does it operate via a brutal form of monopoly-capitalist business practice? (p. 15). Global Hollywood maintains that Hollywoods global authority is due to the clout of its allocation, legal, and economic structures, as opposed to a combination of advantages resultant from the diversity of its domestic audience and its narrative transparency. As this argument has been frequently made by proponents of the cultural imperialism thesis, Miller and his colleagues take a fresh approach that focuses on what they call occasionality (p. 13), which is defined as the specific `uptake of a text by a community (p. 177). Amongst other innovations, the authors focus on the role of audience, and on the idea of rights, while bringing the significant issue of cultural hybridist to political economic analysis. In the short space of twenty five years somewhat which started as US defense inventiveness has developed into the major communications means for the academic and investigates community and most newly has prolonged into a main business tool for the marketable sector. The Internet has developed throughout this period from being a vigorous and effectual way of exchanging information to offering a delivery means for immense amounts of multimedia information to a global audience. While individuals began to use the global media for worldwide communication, its profound effect on how we treat information transfer, organization, and development could not have been anticipated. Internet communication applications permit rapid and simple copy, revision, and transfer of information in textual, visual, and auditory forms. Though the assortments of participants who access it do not all the time agree on whether information must be cosseted or shared, the majority of the Internet community uses, copies, and transfers the information there without restraint. The Internet is a medium for activating ideological consideration; World Wide Web (Web) documents holding multiple links to diverse authors sites as well as e-mail posts restraining various writers materials reify the theory that knowledge is raised from numerous sources. But commercial units that use the Internet to promote products and spend in the materials that they load to the Web desire to keep their digitized materials from copy, revision, and transfer. The corporal operation of the Internet forms a forum where oppositional views concerning control of information collide. The extreme nature of the Internet supports a clash between the constructionist ideology that symbolizes the academic humanist community and the Romantic beliefs that symbolizes traditional legal community. This junction amongst humanistic studies, the intellectual property law, and the Internet, joined with their attendant communities, engenders conflicts in thought and exploit and offers a generous basis from which to investigate intellectual property and information control. Though participants in humanist, legal, and global media communities retain varied ideological beliefs and goals, their common interests meet in forming and treating communicative terms, whether textual, digital, or auditory. More significant, these communities of participants, communally, through socially raised ideologies, contribute in creating approaches toward authorship, possession, and property, and eventually, in generating the power to form and manage knowledge. The dealings amongst these areas can be viewed practically and hypothetically. Globalization, therefore, can tell us diverse stories of the nation state, developing it are relationally and challenged internal and external boundaries. There would be few people concerned in globalization who would, as Green (1997:157) seems to propose, believe that ‘the nation state was disappearing’, even if it’s taken-for-granted status comes to be issued and attempts at self-reproduction become increasingly transparent. The spatial-temporal location of the nation-state is itself brought to the fore by globalization. Globalization is frequently taken to have a single course or logic that results in an augmented uniformity transversely the globe. However, despite the influential effects of international capital and international media corporations, this is not sustainable and is not the stance adopted here. To presume that globalization is about, or results in, homogenization is to abridge the processes at work and, in a sense, to distance oneself from the very composite effects on space, place and uniqueness that globalizing processes bring to the fore. As Giddens (1990) among others suggests, as globalization has resulted in the spread of ‘Western’ institutions across the globe, that very drift produces a pressure for local independence and identity. In other words, globalization is concerning examining places as concurrently traversed by the global and local in ways that have been strengthened by the modern compression of space and time. Thus, alongside the global accessibility of satellite television, McDonald’s and Arnold Schwarznegger films, there is the confirmation of, for instance, local, regional and ethnic identities. Certainly, some transnational companies have overtly adopted strategies of ‘globalization’, expanding their influence around the globe, as situating themselves and their products and services within the local conditions. These might be a response to global influences, but they are however part of globalization and not a refutation of it. What this suggests is that in modern times the local is as much a condition for globalization as the global; space and place are negotiated by the global-local nexus of globalizes space-time compressions. ‘Time-space distanciation, disembedding, and reflexivity mean that composite relationships develop between local activities and communication across distances’ (Waters 1995:50). The assimilation of the globe reconfigures rather than supersedes diversity. Globalization ‘does not essentially imply homogenization or integration. Globalization simply implies greater connectedness and de-territorialisation’ (Waters 1995:136). This problematisation argues that a particular Eurocentric culture can no longer be measured an ‘authentic, self-evident and true universal culture in which all the world’s people ought to believe’ (Lemert 1997:22)—a position which of course itself would not command universal acquiesce. The cultural renaissance resultant from decolonization is the new face of autonomy in international law. Old definitions of freedom focusing on ethnic separation and tight territorial boundaries are becoming ever more outdated. The most interesting and pioneering ideas concerning self-determination are presently being developed by indigenous peoples. Theoretical discussions of prejudice, identity, individuality and universalism might seem remote and incoherent from harsh realities. But these debates do reveal why human rights themselves can spell awful trouble for indigenous peoples. The effects of human rights, intellectual property, transformation and self-determination based on evidently universal ideas of individuality and nationality can consequence in the death of indigenous communities. This is not a current phenomenon. It is the experience of colonization for too many people. And yet, international human rights discourse can also give a mechanism for anti-colonial struggles and the protection of indigenous rights, as the UN Working Group on Indigenous Populations would certainly support. Nowhere is the inconsistency of human rights, culture and individualism as explicit as it is with the rights of indigenous peoples. Moreover, the practical view offers questions and answers to the nuts and bolts of each day treatment of intellectual property power issues. Though interpretive in nature, the practical deportment is rule-based, centered in issues concerning the assortment of original works noted under the law and formative infringement of copyright. An extensive variety of individuals use and produce copyrighted materials in their daily work, often ignorant of the consequences of their actions for probable infringement of the work of others or infringement by others of their own work. Engineers, technical communicators, computer scientists, architects, scientists, and educators, among others who characterizes our diverse national workforce, use and turn out intellectual products such as manual, applications, progress reports, yearly reports, analytical reports, and other technical documents. They as well form non-textual informational materials such as photographs and hand drawn graphics, software, videos, and multimedia products. Additionally, numerous creators acquire information through the global media, together with digital communications such as e-mail and data blocks, as well as graphics, video clips, and sound bytes. Workplace inventors might not be conscious of the special category of law that restrains the rights in the work they turn out. Equally agency laws and the â€Å"work for hire† set of guidelines, which falls under copyright law, state writers rights to their work and treat questions explicit to employees. Educators, particularly, are facing ever more intricate questions concerning forming and using materials for teaching. besides creating workplace products, educators also develop materials for classes in the forms of instructor package that comprise works copied from anthologies and journals, handouts, tests, and instructional transparencies or websites that might be derived from sources formed by other instructors or authors in their fields. The legal argument over what is considered infringement in using these â€Å"course packets† is massive. Instructors might also covet to use materials acquired from the global media. The customary treatment of global media sources as â€Å"free use† forms fussy questions concerning what constitutes infringement in the digital ground. There is also enduring debate over the capability of a browser merely to access a World Wide Web site devoid of infringement. Several legal analysts indicate that the National Information Infrastructures White Paper comprises language that, if construed closely, would forbid admittance to intellectual property on the Internet although the same intellectual property would be available if it were in the shape of print media. For instance, a stringent interpretation of the National Information Infrastructures (NII) White Paper would forbid the mere act of opening a file and reading it on the Internet as the act of producing text in digitized form needs making a â€Å"copy† of the original work. Though the White Paper was formed in 1996, its protectionist stance echoed in legislative development of copyright protection, wherein the No Electronic Theft Act (1998) criminalizes copyright violation and the Sonny Bono Copyright Extension Act (1999) expands copyright protection for a further twenty years. In light of the more and more preventive treatment of copyrighted materials, instructors might be confused over whether they can make non-infringing uses of World Wide Web materials for classroom uses at all (Strong, William S. 1990). Increasingly, numerous instructors inquire students to copy and develop sources procured from the Internet, such as interactions from UseNet News, Internet Relay Chat, and MOOs, and graphics or text files that they can download from the World Wide Web. Though fair use does not converse directly to questions concerning the Internet, it still controls questions of infringement within educational settings. Courts should instigate to apply fair use to issues that are convoluted by use of technology to give new instruction, but until then, prospective litigants looking for answers to complex legal questions must gain a clear considerate of existing law as the best means to recognize its possible interpretation in cases treating issues concerning the Internet. We can say that with the increasing use of internet the issue of Copyright infringement is also become very common. â€Å"Infringement is a breach of the rights of a copyright holder by copying, performing, publishing, displaying, or creating a copied work from an expression protected under copyright† (Strong, William S. 1990). Infringement can take the form of a photocopy, scanned digitization, or other mechanically formed copy, but it can as well take place in videotape, audiotape, performance, or exhibit of a copyrighted work. Providing evidence infringement is at times a complex process, needing that the belligerent party first found a right to control the copyright of the work, then that he or she proves that the work has been infringed. Infringement is further hard to prove while the accused infringer has distorted the work to such a degree that it is hard to sustain the considerable similarity argument and while the initiative and the expression are so wholly merged that use of the idea, which is obtainable in public domain, is corresponding to use of the expression. A more widespread defense aligned a claim of infringement; however, is the scenes a faire principle, which argues that general means of expression of ideas cannot be infringement of anothers work. A typical example is the formal report format used in technical documents. In this case, the means of expression has turn so widespread to the business worlds cultural scaffold of understanding that its use summons connotative expression itself, much similar to a classification of â€Å"technical report. † Copyright infringement elevates legal issues for Internet service providers as well as other global media caught up in network management. The law emerges to be moving away from strict accountability toward a new typical of actual knowledge (Packard, 1998). In the Digital Millennium Copyright Act, ISPs are not legally responsible for copyright infringement if the bringer does not have definite knowledge that the material or an activity using the material on the system or network is infringing (Copyright Law of the United States of America and Related Laws Contained in Title 17 of the United States Code, Circular 92 Pub. L. 105 304, Sec. 512 [c]). Though, upon attaining such knowledge or wakefulness, the provider should act expeditiously to eliminate, or hinder access to, the material. This stipulation has free-expression insinuations. Copyright law is a moderately recent phenomenon based on the supposition that inspired intellectual property desires to be protected and rewarded (Packard, 1998). By distinguishing that online services cannot scrutinize their content for infringing material and function professionally, Congress has given them a green light to expand to their full prospective (p. 37). The copyright extension for elite ownership for ninety-five years, up from twenty-eight years in the original 1790 law, has been dared in court by Eldritch Press. Under the new law, the publisher would be requisite to eradicate work that has been in the public domain under the preceding limit of seventy-five years. The global media and its technologies have offered fertile view for the creation of new communication technologies. Inventors functioning on such troubles as digital compression as well as network data-transfer speeds need patent protection to be capable to expand new products. Information technology has also taken a diversity of patent suits as inventors extend the new industry. Lucent Technologies, for example, sued Cisco Systems and indicted it of infringing eight digital networking patents. Cisco then charged that Lucent violated three of its patents. Lucent holds thousands of patents on former Bell Lab and ATT research operations, and analysts feared that the aggressive action by Lucent was threatening to smaller high-tech companies. Computer-chip giant Intel called a patent infringement action by TechSearch a nuisance lawsuit (Packard, 1998). As technology continues to become more multifaceted and consistent, patent disputes are probable to propagate. Generally, most patent cases do not have a substantive collision on free expression. Thus the main features of the global media regime are linked to infringement and intellectual property concerns. The strategy for these aspects of the establishment is the principle that the costs of Internet-related infrastructural development shall be borne mainly by the private sector and the standard those governments shall entrust themselves to economic liberalization, privatization, and regulatory programs dependable with this and other regime principles. As the utmost basis of legal conflict is that between authors and users rights, the most significant policy issue is cared for specifically in the Constitutions intellectual property stipulation. The goal of the copyright act is to make sure free speech and the progression of knowledge through our legitimate protection of the right to distribute information. The unique constitutional provisions designate the intent to make sure the expansion of knowledge in civilization based in a congressional grant to authors of a partial monopoly of rights in their works: The fair use stipulation makes clear that the key goal of the statute is to support learning. These changes notwithstanding, the divergence between authors rights and the goal to encourage knowledge, inner to the copyright debate since its setting up, continues. Sadly, the public policy issue is frequently ignored in respect to concerns over economic interests. The everyday application of law essentially focuses on treating conflict between individuals. Lawyers are trained specially to congregate the needs of the legal system and are inexpensively supported by their work in this area. However the policy issues following the statute are really most significant to us as educators and to our society as a whole because those who manage the development of knowledge in a culture eventually establish who we are as a people. Philosophy and the goals that convoy it drive our view of policy issues. Thought determines how we view authorship, possession, and property and eventually affects not only how intellectual property law is proscribed but how information and communication that are inner to the dialogic processes within the nation are proscribed, as well as decisive who controls them. An assessment of ideological choices in request to intellectual property thus renders significant understanding of the probable effect of the law on our cultural future. Gaining a considerate of intellectual property issues is inner to understanding our rights as users and producers of knowledge. The actions we acquire to influence egalitarian access to information can have enduring ramifications for society, as authorship makes control, control generates authority, and authority generates power. We must take every step needed to ensure that the controlling voices of the few but authoritative are reasonable by the yet-unheard voices of the weaker multitudes. Reference: Baym N. K. 1995. The emergence of community in computer-mediated communication. In S. G. Jones, ed. , CyberSociety: Computer-Mediated Communication and Community. Thousand Oaks, Calif. : Sage Publications, pp. 13863. Buck K. 1996. Community organizing and the Internet. Neighborhood Works, 19, 2, p. 2. Copyright Law of the United States of America and Related Laws Contained in Title 17 of the United States Code, Circular 92 Pub. L. 105 304, Sec. 512 [c] Gates B. 1995. The Road Ahead. New York: Viking Giddens, A. (1990) The Consequences of Modernity, Cambridge: Polity Press. Green, A. (1997) Education, Globalisation and the Nation State, London: Macmillan. http://www. washingtonwatchdog. org/rtk/documents/cong_hearings/senate/107/senatehearing107_77094. html http://www/stephenking. com Laikwan Pang`s 2005 article `Copying Kill Bill` social Text, No. 83, 133-153. London Times, June 17, 1996. Packard A. (1998). Infringement or impingement: Carving out an actual knowledge defense for sysops facing strict liability, Journalism Mass Communication Monographs, no. 168 (December). Ratan S. 1995. Time (spring):25-26. Rheingold H. 1993. The Virtual Community: Homesteading on the Electronic Frontier. Reading, Mass. : Addison-Wesley. Strong, William S. The Copyright Book: A Practical Guide. Cambridge: MIT P, 1990. Toby Miller et. al, 2001 `Hollywood`s Global Rights` in Toby Miller et. al. , Global

Cell Culture and Proliferations | Experiment

Cell Culture and Proliferations | Experiment To study the cell proliferation of Mouse Y1 adrenocotical cells by using MTT (3-(4, 5-diphenyl tetrazolium bromide) and crystal violet staining methods. INTRODUCTION In general, Cell proliferation means multiplication/Reproduction of cells for increasing cell population in a very short span of time. The assay of cell proliferation is to measure the number of cells which are present in the dividing culture medium. Cell proliferation is controlled by using growth factor (Fetal calf serum), Which normally bind to the surface receptors present on the cell membrane generally regulate the cell signaling molecules, which pass the message to nucleus by help of receptor generally where the transcription factor gets bind to the DNA, makes turn off turn on the protein synthesis mechanism, responsible for cell division. Cell proliferation method is very important for studying various biological factors like bioassay, carcinogenic analysis and other toxicological tests. Generally two metods are used for studying cell proliferation i.e. crystal violet staining method and MTT (3-(4, 5-diphenyl tetrazolium bromide) method, here these methods are used to study ce ll growth in mouse Y1 adrenocotical cells. In crystal violet staining method, the DNA of cells is going to stained by crystal violet which produces a colour intensity that is proportional to the cultured cells(including newly proliferated cells). In this method, the principle involved to calculate the cell proliferation is based on the absorbance taken up by the viable cells in culture at different concentrations after the cells are stained with crystal violet. In MTT (3-(4, 5-diphenyl tetrazolium bromide) method, the assay depends on the amount of MTT taken up by the cells, tetrazolium salt is water soluble which produces yellow colour. The tetrazolium MTT is metabolically reduced by active cells, in presence of dehydrogenase enzymes, producing NADH and NADPH which are reducing equivalents. This results in the formation of purple formazan intracellularly, which is measured by spectrophotometer. MATERIALS METHODS Cell culture: In DMEM (Dulbeccos modification of eagles medium),mouse Y1 adrenocotical cells which were grown on monolayer was removed by using mixture of trypsin and EDTA (0.05% and 0.02% ).The cells are incubated by 5minutes by adding Trypsin/EDTA(7ml).After incubation the flask was removed and tapped gently to separate undetached cells. The contents of the cell are transferred to a universal container for centrifugation at 1000rpm/ 5mins.Supernatant was discarded and medium is added for resuspending the cell pellet. The cell number was estimated by using Haemocytometer counter for 15ml the cell suspension dilution was prepared containing density 1.25X105 cells/ml of suspension. In 96 well plate, 60 wells were filled with 100Â µl cell suspension, in this the remaining outer wells are filled with Phosphate Buffer Solution (PBS) of 200Â µl.Allowed the plate overnight in a gas incubator to settle down the cells. Those cells were treated with Fetal Calf Serum (FCS) of different concentrations vary ing from 0% to20% in universal tubes.12 wells of plate were filled with 200Â µl of different concentrations. These plates were incubated for a period of 24hours. Cells were washed with Phosphate Buffered Solution (PBS) for three times using multichannel micropipette; later media containing various serum concentrations were added. These two 96 well plates were used for crystal violet staining and MTT (3-(4, 5-diphenyl tetrazolium bromide) assay after 72 hours of incubation. Crystal Violet Staining Method: The cells were removed from central 60 wells of 96 well plates and filled with 200Â µl of methanol in a fume cupboard. After 15minutes methanol was removed from the plate and was left to dry in the fume cupboard. Once the plates were dried the cells were stained with 200Â µl of crystal violet. Once the staining is complete after 20minutes the plates were washed with Distilled water for atleast three (3) times and then solubilized the cell layer by using 50Â µl of 10% glacial acetic acid. The plates were then kept for incubation in a gas incubator for thirty minutes after incubation Absorbance of wells was measured at 540nm. MTT STAINING METHOD: The cells present in the central 60 wells of 96 well plate were treated with 20Â µl of MTT (5mg/ml solution in PBS) and was left for 4hours in gas incubator at 370c.After incubation by using multichannel pipette medium was removed and 100Â µl of acid-isopropanol was added in order to dissolve the blue formazan crystals from the cell layer and then it was incubated for 30minutes at room temperature after solubilizing formazan crystals Absorbance was measured at 570nm using plate reader. Calculation: Total No. of cells in 5 square = 21 Average cells present in one square = 4.2 Calculation of cell number: The volume of each square is= 4 X 10-3 The total cell number for 5 square gives the cell = 0.02Â µl No. of cells in 1 ml = 105X104 Number of cells required = 395X104 Volume of suspension required = concentration required/ concentration got = 395X104/105X104 = 3.7619 ml cell suspension to be taken Medium to be taken =30,000Â µl-3.7619Â µl = 29996.23Â µl medium to be taken. DISCUSSION: Here the increase in the absorbance with corresponding to the fetal serum concentrations shows the sign for the cell growth. Ammonium cations bind to negatively charged DNA which in turn gave blue color to the mixture. By using the color intensity, viable cells were estimated by means of haemocytometer. No experiment will produce 100% results. So here also errors occurred due to practical errors. Occurrence of errors might be due to: Washout condition of stained culture cells Improper solubilisation of 10% glacial acetic acid. In MTT method the degradation of MTT gives color to the mixture. This degradation was due to the dehaydrogenases of viable cells. The color intensity is directly proportional to the cell growth. Here also the errors might be occurred due to improper solubilisation of formazan crystals (Butler. 1996), (Javoise. 1998). DIFFERENTIATION OF K562 CELLS TO PLATELETS IN PRESENCE OF PMA: Differentiation of K562 cells to megakaryoctes/platelets Phorbol Myristate Acetate treated and untreated cells were spun down in a bench centrifuge and after resuspended in 1ml PBS (having 1 % Bovine serum albumin). Then by using haemocytometer the cell number was calculated, after diluting the suspension. cytospin was added to 1 ml cell volume which was adjusted to density of 106 cells per ml. In assembled cytospin 200 Â µl at 1000 rpm/3min. After fixing the slide in acetone /methanol(50:50), slide was washed with 0.15 M tris buffered saline .In humid temperature Human cd61 cells were incubated for 2 hrs using TBS slide was washed.with rabbit anti- mouse Ig-G anti body cells incubated for for 30 mins at room temperature and washed with TBS, after washing, cells were incubated with Alkaline phosphate anti-alkaline phosphate complex, this was repeated with Ram and APAP for amplification. They were washed under running tap water after staining with red TR substrate and counter was stained with haemotoxylin. Finally the slide was viewed und er microscope after washing with TBS.PMA is a diester of phorbol and a tumor promoting agent (proc.Natl.Acad.sci.USA Vol.82, pp, 3859-3862, june 1985Medical sciences). PMA initiates the signal transduction by protein kinase C (PKC) enzyme which allows promoting the differentiation of K562 cells.By using CD 61 marker the K562 cells were treated .These CD61gets attached to cell network to work as primary antibodies. In addition with cells performs a seconndary antibobodies whenever exposed to Ram along with APAP and forms pink color by attaching to FC region of anti-human CD 61 antibodies. This phenomenon gives the cells under going differentiation when incubated with PMA (MSc Pharmacology Biotechnology, cell biology laboratory manual/ January 2010) RESULT: The slide treated with Phorbol Myristate acetate (PMA) is in pink colour, whereas the slide which is untreated with Phorbol Myristate acetate (PMA) is in blue colour after staining. The cells when treated with PMA differentiate into Platelets/Megakaryocytes. In PMA the diseter bond promotes the tumor, which in turn activates the signal transduction of protein kinase C enzyme(PKC) inn K562 cells causes the CD61 expression. The RAM IgG gets attached to the CD61 antibodies, these K562 cells when incubated with APAAP form a complex. Later fast red dye was added to the mixture which gets attached to the APAAP, the cells turn pink by taking the stain. The cells containing PMA expressed the CD61. It generates the (signal transduction protein kinase C) PKC enzyme and on of K562 cells causes the expression of CD61. The rabbit antimouse IgG antibodies attach to the antibodies of CD61 when incubated in the presence of APAAP (alkaline phosphatase anti-alkaline phosphatise) on of K562 cells complex. Then we add the fast red dye to the mixture which was attached to APAAP (alkaline phosphatase anti-alkaline phosphatise) and stains the cells pink. CD61 was expressed by only those cells which had PMA (Shelly, 2000)

Barbie - A Complex American Icon Essay examples -- American Culture E

As a young girl, I was not very interested in playing with baby dolls. I preferred playing with my many stuffed animals or the only doll I did like—Barbie. With my animals, usually I was rescuing them from some horrible disaster such as a flood or a forest fire. I was their heroic savior and benevolent protector. But with Barbie this was decidedly not the case. Sometimes my Barbie did normal Barbie things, such as get dressed up for an exciting date with Ken or go shopping with her little sister, Skipper. More often, however, I subjected Barbie to strange, sadistic acts of my imagination. Frequently Barbie, in her pink dune buggy, would have tragic head-on collisions with my brother’s dump truck, or the brakes would suddenly go out on her pink Barbie scooter, sending her careening off a steep mountain cliff. Barbie also had the unfortunate tendency to be sucked from her Barbie plane by her lovely long blonde hair while flying at 30,000 feet. Since in every other way I w as a normal child, psychoanalysts might interpret my play patterns with Barbie as childlike manifestation of women’s frustrations at the disparate images popular culture presents for women. Most women I know also experience this love/hate feeling towards Barbie and the mixed messages she represents, especially when their daughters start begging for Barbies of their own. While mothers do not want to encourage the unrealistic beauty expectations that Barbie represents, they also fondly remember Barbie as their own favorite toy. These many women, and their daughters, have made Barbie the most successful toy for girls since 1959, despite Barbie’s many contradictions. Barbie embodies American popular culture’s attempt to respond to women’s changing roles in the era since... ... â€Å"Barbie is a Million-Dollar Doll,† The Saturday Evening Post, December 12, 1964, 72. 23 Douglas, 24. 24 â€Å"All’s Swell at Mattel,† Time, October 26, 1962, 90. 25 â€Å"It’s not the Doll it’s the Clothes,† Business Week, December 16, 1961, 48. 26 Cleo Shupp, â€Å"Little Girls are too Sexy too Soon,† Saturday Evening Post, June 29, 1963, 12. 27 Zinsser, 73. 28 â€Å"The Barbie-Doll Set,† Nation, April 27, 1964, 407. 29 Donovan Bess, â€Å"The Menace of the Barbie Dolls,† Ramparts, January 25, 1969, 25. 30 quoted in Bess, 26. 31 Letty Pogrebin, â€Å"Toys: Bad News/Good News,† Ms., December 1975, 60. 32 Douglas, 27. 33 Douglas, 25. 34 â€Å"Zeitgeist Barbie,† Harper’s Magazine, August 1990, 20. 35 Helen Cordes, â€Å"What a Doll!,† Utne Reader, March/April 1992, 46. 36 taken from December 2004 Toys R Us, Wal Mart, Target, and K-Mart advertisements.

Motif Affairs: The Great Gatsby

Throughout the novel The Great Gatsby, Fitzgerald uses the motif of affairs to show development in Daisy Buchanan's character. As a reader slowly pieces together what is the love puzzle of this novel, it becomes clear to them Daisy's true self. Starting off the novel Fitzgerald uses Tom's affairs with Daisy(his wife) and Myrtle(his mistress) to show how Tom treats Daisy. He is very sexist towards not only Daisy but also myrtle. â€Å"Daisy loved me when she married me and she loves me now,†(131) this is said by Tom, giving the reader an example of just how controlling he is of his wife.It is evident to the reader, through the lens of feminism, that Tom objectifies women, treating them like objects rather than people, Daisy especially, making him feel it is acceptable to have more than one woman. This gives the reader a sense of sympathy for Daisy, because of the way her husband treats her. It upsets the reader that although Daisy is aware of her husband's affair with Myrtle, s he ‘accepts' it and feels she deserves it because she is a woman and that's simply what women get.As the novel goes on, it is made clear to the reader Daisy's obsession with money. â€Å"Her voice is full of money†¦ That was it. I‘d never understood before. It was full of money – that was the inexhaustible charm that rose and fell in it, the jingle of it, the cymbals‘ song of it†¦ High in a white palace the king‘s daughter, the golden girl†¦ â€Å"(120) We learn of an affair in Daisy's past that she had with Gatsby, and that the only reason she married Tom rather than Gatsby was because of Tom's wealth.When this is brought up it refuels old feelings between the two of them, leading to an affair between Daisy and Gatsby. Readers go from feeling bad for Daisy to almost having sympathy for Tom. After accusing Tom of objectifying Daisy and not being loyal to her, it is made clear that daisy is not loyal to Tom. She is not in love with him, but rather his money. Through these recurring affairs and the lens of feminism Daisy's true character is exposed to the reader.

The novel Jekyll and Hyde

Written in the 1880's by famous British author Robert Louis Stephenson, the novel Jekyll and Hyde is about one man with dissociative personalities. Jekyll and Hyde, although are portrayed to be two very different characters all together, are in fact the same man, wanting to fulfil certain â€Å"evil† pleasures whilst still being a well respected member of society. However both of these desires cannot be overcome without a magic potion created by Dr Jekyll, used to transform himself into the ghastly looking and somewhat evil being, known in this book as Hyde – his other half. In the late 1800's there were a few ideas and theories arising which could be what inspired Stephenson to write such an odd and interesting book. The significant developments in ideas which could be associated with this story are Charles Darwin's theory of evolution and Sigmund Freud's psychological theories on dissociative personalities, which came around at the time of Stephenson writing this book. These could be linked to how Hyde is from an era where there was no such thing as morality, Hyde can also link to Freud's id (animal instinct), ego (what controls us in order to achieve others approval) and superego (sense of self), in this way Jekylls id takes over. Stephenson could also be proving a point about the class system at that time in the UK as there was a large class division, where as Jekyll has a rich, well off posh lifestyle and Hyde is a character who is â€Å"crude† and appears to be of a lower class, not at all respected in the society in which he lives. The first example of Stephenson portraying Hyde as being evil comes midway though the first chapter where he is first described as â€Å"a little man stumping along. † Already he is portrayed to have an ugly negative appearance, and his physical descriptions are one of the main ways which Stephenson describes Hyde overall. Next he is shown to knock over a small child in the street, and one may assume this was an accident, and it most likely was, however when Hyde shows no affection to the girl he just knocked over, and literally tramples over her, the audience knows he is a bad character. Mr Enfield (a friend of the lawyer; Mr Utterson) after seeing this describes the incident as â€Å"hellish† and Hyde as â€Å"a damned juggernaut†, which relates Hyde to becoming violent as the Indian God of war known as Jaggernafth. Here he is also compared to Satan, which refers him to evil yet again. Another way in which Stephenson portrays Hyde to be evil is through his physical descriptions, however there is a vagueness of his facial descriptions due to the fact other people find him indescribably ugly. In chapter 8 he is said by Poole (Jekylls butler) to â€Å"have a mask upon his face† as such an appearance is too inhuman and unnatural to be that of a normal man. Also in that chapter Hyde is commonly referred to as â€Å"it† rather than â€Å"him† which suggest he has a lack of humanity. He is also called a â€Å"creature† and described as having a dismal screech, as of mere animal terror. This creates a sense of animal imagery, which is one of the ways that Stephenson portrays Hyde as being evil. Hyde's clothes are described to be far too big, and that is because they are the exact same clothes worn by Dr Jekyll. This therefore shows that Jekyll is considerably taller than Hyde, and Stephenson here could be saying that not only does Jekyll overshadow Hyde in a physical sense, but also in the sense that the good in Jekyll is much greater than the evil in Jekyll. Dr Jekyll is a rather interesting character who wants and respects his good reputation, but still craves some of life's somewhat quirky pleasures. Should we question if Jekyll is as morally good as people, such as his close friends think he is, or if he is simply a scientist with urges to fulfil? In chapter ten Jekyll describes his â€Å"duplicity† and he also describes his first feelings of his â€Å"new life† as Hyde. After the â€Å"racking pangs† of the initial transformations, Jekyll describes himself as feeling â€Å"indescribably new† and â€Å"incredibly sweet†. He feels â€Å"younger, lighter happier in body† and he also talks about the â€Å"freedom† of his soul, what is also interesting is how he felt â€Å"wicked† yet delighted. This is the first point in which the audience know Jekyll and Hyde are in fact one, but also that Jekyll enjoys his transformation from good to evil. This idea of Jekyll enjoying his transformation suggests that maybe Jekyll is actually not so morally good. Also Jekyll describing his † dual nature† and saying â€Å"I concealed my pleasures† also go to show maybe Jekyll isn't the most morally good character which goes beside other character opinions on him such as Dr Lanyon's â€Å"He began to go wrong in the mind. He does however get more pleasing and positive descriptions at the start of the story, where Mr Utterson compliments him, saying he is a â€Å"well-made, smooth faced man† who throws â€Å"pleasant dinners† and who's personality was one of sincerity. As well as himself, his house is described as â€Å"comfortable† & â€Å"warmed† by Utterson which could reflect Dr Jekyll as a person. On top of this, the denotations â€Å"F. R . S. are next to his name, which stand for Fellow of the Royal Society, and to be in such a highly respected club portrays Jekyll as a man with a much esteemed reputation and a man of high intellect. These qualities of Jekyll alongside others like his â€Å"nature to industry† and â€Å"high head† that when put against his dark secrets and new good feelings after his first transformation, show that Jekyll is generally good but is only human so he will have his secrets, which as deep as they may be, show that he is not evil, but nobodies perfect? From reading this book, it is clear to see that Stephenson uses several ways to show the true personalities of Jekyll and Hyde. The first way in which Hyde is depicted to be evil is through the descriptions of his actions, in Chapter one he is described to be stumping along and this creates a sense of negativity rather than just walking. The violence and severity of the verbs Stephenson uses also contribute to the portrayal of Hyde's evil nature. He also uses animal imagery such as â€Å"ape-like fury† which compares him to an animal, a creature with no morals, and of a lower class. Then there is Jekyll, who Stephenson portrays to be the complete opposite of Hyde, and does this by talking about his privileged background, good stature and good looks, and also gives him the title Dr rather than Mr, as anyone would think of a Dr as a well respected and highly intellectual member of society.